《植物生理学报》 2017, 53(8): 1359-1364

多酚氧化酶催化合成茶黄素研究

林彩霞, 杨静蕊, 王光瑛, 倪贺^*, 李海航

华南师范大学生命科学学院广东省植物发育生物工程重点实验室, 广州510631

通信作者：倪；E-mail: 022524328@163.com

摘　要：

茶黄素是红茶的主要品质成分, 并具有广泛的生理药理活性。利用多酚氧化酶(polyphenol oxidase, PPO)体外酶促转化是生产茶黄素的有效方法。本文从PPO酶源的筛选、反应体系的优化、固定化PPO转化茶黄素及PPO构效关系等方面综述了PPO催化合成茶黄素的研究进展, 为工业化制备茶黄素奠定基础。

关键词：茶黄素; 多酚氧化酶; 固定化; 催化合成

收稿：2017-04-05   修定：2017-08-01

资助：广东省自然科学基金(2014A030313423)、广东省科技计划项目(2014A010107026)和国家自然科学基金(31401660)。

Catalytic synthesis of theaflavin with polyphenol oxidase

LIN Cai-Xia, YANG Jing-Rui, WANG Guang-Ying, NI He^*, LI Hai-Hang

Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China

Corresponding author: NI He; E-mail: 022524328@163.com

Abstract:

Theaflavin is the main component of black tea, having various biological activities. Production of theaflavin by polyphenol oxidase (PPO) in vitro is deemed to the best method. In this review, screening of PPOsource, optimization of reaction system, synthesis of theaflavin with immobilized PPO, and structure-activity relationship of PPO were summarized, which provided theory and technology supports for preparation of theaflavin in industry.

Key words: theaflavin; polyphenol oxidase; immobilization; catalytic synthesis